GSM4286170: AM2045-8_S8: Low-fat rep4; Mus musculus; RNA-Seq - SRA

SRX7638016: GSM4286170: AM2045-8_S8: Low-fat rep4; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 86.3M spots, 8.6G bases, 3.6Gb downloads

Submitted by: NCBI (GEO)

Study: Transcriptomics analysis of lineage-restricted Dendritic-cell (DC) progenitor cells in obesity

PRJNA603380 • SRP245437 • All experiments • All runs

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The overall goal of the project is to understand transcript-level changes in DC-progenitors from bone marrow of diet-induced obese mice by Next Generation Sequencing (NGS; RNASeq) Overall design: DC-restricted progenitors (Lin-/c-kit(int)/Sca1(lo)/CD115+/Flt3+; ~50,000 cells) from bone marrow (BM) compartment of high fat (60kcal % fat) & control chow (10 kcal%fat)-fed mic (n=4 per group) were flow-sorted and RNA was isolated. The whole transcriptome was amplified and library was constructed using NuGen Ovation Solo RNASeq System; and evaluated on high-sensitivity DNAchip (Agilent) and sequenced on a HiSeq 3000 platform

Sample: AM2045-8_S8: Low-fat rep4

SAMN13930507 • SRS6068592 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: DC-restricted progenitors were flow-sorted directly into lysis buffer and RNA was harvested using Arctus PicoPure RNA isolation kit, quality tested by Bioanalyzer (Agilent)for the construction of sequencing libraries. RNA libraries were prepared for sequencing using NuGen Ovation Solo RNA-Seq System (Mouse part no 0501) protocols

Experiment attributes:

GEO Accession: GSM4286170

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 86.3M spots, 8.6G bases, 3.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR10972547	86,283,287	8.6G	3.6Gb	2020-02-04

ID:: 9967568

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